Genome-Scale CRISPR-Cas 9 Knockout Screening in Human Cells
Introduction The CRISPR-Cas9 system, which can be programmed to target specific genomic loci (position), offers a powerful tool for gene editing and functional genomics. This study demonstrates developing and applying a genome-scale CRISPR-Cas9 knockout (GeCKO) library for high-throughput screening (the use of automated equipment to rapidly test thousands to millions of samples for biological activity […]
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