Genome-Scale Functional Genomics Using CRISPR-Cas9: Advancements, Challenges, and Applications in Identifying Essential Genes
Introduction The CRISPR-Cas9 system represents a groundbreaking advance in gene editing and functional genomics, allowing scientists to target and modify specific genomic loci with precision. This method, which uses guide RNA (gRNA) sequences to direct Cas9 endonuclease activity, can induce double-strand breaks in DNA, which are then repaired by cellular mechanisms that often result in […]