This is a scientific literature review on the article Muscle Stem Cell-Derived Extracellular Vesicles Reverse Hydrogen Peroxide-Induced Mitochondrial Dysfunction in Mouse Myotubes which you can find here https://pubmed.ncbi.nlm.nih.gov/33256005/ The experiment was a long one, so I decided to split the review up into two parts; that way, it won’t be such a long read. The […]
Introduction: A Revolutionary Step in Genetic Science For decades, scientists have dreamed of having the ability to modify genes with precision, to go into the very blueprint of life and make changes that could cure diseases, strengthen crops, and even potentially shape the evolution of living organisms. Yet, for most of history, genetic engineering was
In this post, I will be presenting my scientific literature review of the following article: https://pmc.ncbi.nlm.nih.gov/articles/PMC11078688/ Article Reviewed:The Use of CRISPR-Cas9 Genetic Technology in Cardiovascular Disease: A Comprehensive Review of Current Progress and Future Prospective. Authors:Asif M, Khan WJ, Aslam S, Aslam A, Chowdhury MA. Introduction and Background This review outlines the transformative journey of
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Introduction The CRISPR-Cas9 system represents a groundbreaking advance in gene editing and functional genomics, allowing scientists to target and modify specific genomic loci with precision. This method, which uses guide RNA (gRNA) sequences to direct Cas9 endonuclease activity, can induce double-strand breaks in DNA, which are then repaired by cellular mechanisms that often result in
Background: The experiment conducted in this research article explored the possibilities of using muscle stem cells to repair damaged and dysfunctional muscles. This type of therapy could be a form of treatment for people with muscular dystrophy. The disease that was focused on here was Duchenne muscular dystrophy (DMD). To understand the aim of this
The paper introduces CRISPR-Cas systems as small RNA-based defense systems that execute adaptive and heritable immunity against viruses, plasmids, and other mobile genetic elements in archaea and bacteria. The system utilizes CAS proteins, associated with the loci of the CRISPRs in recognizing and destroying the invasive nucleic acids. The components that supply this immunity mediated
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Introduction The CRISPR-Cas9 system, which can be programmed to target specific genomic loci (position), offers a powerful tool for gene editing and functional genomics. This study demonstrates developing and applying a genome-scale CRISPR-Cas9 knockout (GeCKO) library for high-throughput screening (the use of automated equipment to rapidly test thousands to millions of samples for biological activity
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Overview of CRISPR CRISPR is a technology that enables researchers to edit parts of a genome. It works via the enzyme Cas9 and a guide RNA strand. Cas9 acts as a pair of ‘molecular scissors’ that can cut the two strands of DNA at a specific location. Guided RNA is designed to find and bind
